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Molecular Biology

PCR not amplifying DNA

Why Is My PCR Not Amplifying DNA?

Table of Contents Polymerase Chain Reaction is one of the most widely used molecular biology techniques for DNA analysis, diagnostics and research. However, there are times when even professionals encounter situations where the PCR is not amplifying DNA as expected. A failed reaction may delay experiments, increase costs, and affect the reliability of the results. …

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gel-electrophoresis

Gel Electrophoresis: DNA vs RNA vs Protein Differences Explained

Gel electrophoresis results in the separation of DNA, RNA, and proteins based on different principles. DNA and RNA separation takes place based on size by using agarose or polyacrylamide gels. On the other hand, protein separation occurs based on size and charge using SDS-PAGE or native PAGE. Each method uses specific buffers, stains, and conditions …

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probe_sonicator

How to Choose the Right Probe Sonicator for Genomics and Proteomics Labs?

For researchers trying to extract high-quality nucleic acids and proteins, the one tool that they need is the probe sonicator. It is widely used for fragmentation of DNA for sequencing, shearing chromatin for ChIP assays, and extracting proteins for mass spectrometry. However, choosing the right equipment can put any buyer in a dilemma when it …

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