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PCR not amplifying DNA

Why Is My PCR Not Amplifying DNA?

Table of Contents Polymerase Chain Reaction is one of the most widely used molecular biology techniques for DNA analysis, diagnostics and research. However, there are times when even professionals encounter situations where the PCR is not amplifying DNA as expected. A failed reaction may delay experiments, increase costs, and affect the reliability of the results.

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Biosafety Cabinet Class

How to Choose the Right Biosafety Cabinet for Your Laboratory: Complete Buying Guide?

A biosafety cabinet is a ventilated enclosure used in laboratories and designed to protect laboratory personnel, research samples, and the surrounding environment from biological contamination. The best equipment for labs depends on the type of research, biosafety level, airflow requirements, and the category of cabinet. Laboratories working with infectious agents, cell cultures, pharmaceuticals, and microbiological

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hepa_filter_efficiency

What Level of HEPA Filter Efficiency is Used in Laminar Air Flow Systems?

Laminar air flow systems typically use HEPA filters with an efficiency of 99.97% at 0.3 microns. This implies that the filter can remove about 99.97% of airborne particles that are 0.3 micrometers, which is considered the most penetrating particle size. Laminar air flow systems are an integral part of laboratories, manufacturing units of pharmaceutical companies,

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pcr_workstation_nabl_img

Is A PCR Workstation Mandatory for NABL-Accredited Labs?

A PCR workstation is not legally mandatory for NABL-accredited laboratories. However, it is highly recommended for labs performing PCR-based molecular testing. According to guidelines of NABL and ISO15189, laboratories need to maintain contamination-controlled environments for molecular diagnostics. A PCR workstation NABL help meet these conditions, making it an important component in many in several NABL-compliant

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lab_drying_methods

Which Lab Drying Method Best Preserves Biological Samples?

Preserving biological samples during drying is a critical step in diagnostics and research labs. Choosing a wrong drying method can degrade RNA or DNA and compromise the sample integrity. Here is a comparison of common lab drying methods and explains which option is best for sensitivity, tolerance, and research goals: Why drying Methods Matter for

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laminar_air_flow_bench

How Laminar Air Flow Is Used in Tissue Culture Labs: Step-by-Step Process

How do laminar airflow cabinets work in tissue culture labs? Learn a step-by-step process to maintain sterility, prevent contamination, and improve lab safety.  A laminar air flow cabinet is widely used in tissue culture laboratories to maintain a sterile working environment. It works by passing air through HEPA filters to remove dust, contaminants, and microbes

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iGene - Biosafety Cabinet Class II B2 - Image Creations

Common Reasons Biosafety Cabinets Fail Containment Tests and How to Fix Them

Table of Contents Biosafety cabinets fail containment tests due to improper airflow balance, damaged HEPA filters, poor installation, or inadequate maintenance. Regular certification, proper placement, and timely replacement of filters help restore the performance of the equipment and ensure safe handling of hazardous biological materials in laboratories. A BSC is one of the most crucial

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Power Supply IG-4006PS

How to Choose an Electrophoresis Power Supply for Molecular Biology Labs?

Electrophoresis is one of the basic techniques in molecular biology laboratories used to separate DNA, RNA, and proteins based on size and charge. While gels, buffers, and chambers receive maximum attention, power supply is that component that directly controls accuracy, reproducibility, and safety. Read this guide to understand the significance of power supply for electrophoresis

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gel-electrophoresis

Gel Electrophoresis: DNA vs RNA vs Protein Differences Explained

Gel electrophoresis results in the separation of DNA, RNA, and proteins based on different principles. DNA and RNA separation takes place based on size by using agarose or polyacrylamide gels. On the other hand, protein separation occurs based on size and charge using SDS-PAGE or native PAGE. Each method uses specific buffers, stains, and conditions

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